Environmental Monitoring of Food- and Waterborne Parasites (PARASITE HUNTER)

Project leader: Dr. Ivana Klun

Project collaborators: Dr. Duško Ćirović, Dr. Oliver Tošković, Dr. Aleksandra Penezić, Dr. Stanislav Simin, Dr. Aleksandra Uzelac, Vladimir Ćirković, Dragana Miličić, Nikola Betić, Neda Bogdanović, Ilija Pantelić

Project number: 2424

Duration of the project: 2024-2026.

Leading institution: Institute for Medical Research, University of Belgrade

Collaborating institutions: Faculty of Biology, University of Belgrade, Faculty of Philosophy, University of Belgrade, Faculty of Agriculture, University of Novi Sad, Institute of Meat Hygiene and Technology

Description of the project:

The increasing prevalence of food and waterborne parasitoses in humans and animals in Europe calls for the need for holistic surveillance to detect emerging threats and identify reservoirs of infection, so that public health authorities can be alerted in a timely manner. Systematic monitoring programs for most of the parasites which cause these diseases do not exist and most parasitoses are not notifiable, thus it is upon the scientific community to gather evidence of these silent epidemics, raise awareness and advocate for innovative and improved food safety measures to reduce disease burden.

PARASITE HUNTER aims to incorporate One Health principles in food safety, with a strong emphasis on environmental matrices, as the 21st century is characterized by significantly perturbed ecosystems due to anthropogenic disturbances. The impact of ecosystem health and wildlife on food safety will be evaluated and communication with stakeholders will be established. Standardized parasitological methods [sedimentation, (Mini)FLOTAC] and validated molecular techniques (multiplex qPCR, nested PCR) will be applied to detect, quantify, and assess viability (staining and in vitro culture) of food and waterborne parasites (FWP) of public health significance in various human and animal foods, water and soil in which the food is produced. This project aims to establish a data driven surveillance framework for FWP to improve food safety beyond the current standard by including monitoring of vulnerable groups (humans and animals) not covered by the current food safety legislation. Stakeholders will be engaged through questionnaires to assess the level of awareness regarding FWP and help draft a white paper with proposals to reduce/control the FWP burden. As a result of the project activities, a sustainable, state of the art FWP surveillance program for timely identification of emerging threats to public health, and stakeholder approved measures to improve food safety are expected.

Тип пројекта:

National Projects

Worm Profiler: Surveillance and Population Genetics of Echinococcus in Serbia

Project leader: Dr. Aleksandra Uzelac

Project collaborators: Dr. Katarina Breka, Dr. Jelena Karanović, Dr. Nikola Betić, Dr. Milica Kuručki, Dr. Tijana Kukurić

Project number: 10841

Duration of the project: 2024-2026.

Leading institution: Institute for Medical Research, University of Belgrade

Collaborating institutions: Faculty of Biology, University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Institute of Meat Hygiene and Technology, Faculty of Agriculture, University of Novi Sad

Description of the project:

Analysis of the population genetics of Echinococcus is an ongoing effort in some parts of Europe, while the Balkans represent a significant knowledge gap. This project proposes to comprehensively survey the entire transmission cycle consisting of intermediate and definitive animal hosts and the environment using sample processing and analytical methods which have been standardized, validated and harmonized at the EU level to obtain high quality population genetics data via mitochondrial gene (cox1 and nad1) sequencing and characterization of the EmsB microsatellite from single eggs, larvae and adults. Specifically, this approach allows for the survey of as of yet underexplored reservoirs with a potentially high transmission capacity to humans. The population genetics data along with GPS coordinates of identified infected hosts will be systematized and graphically displayed through an interactive bioinformatics database, WormProfiler, to help identify transmission foci and ultimately model transmission dynamics. WormProfiler’s user interface will be specifically tailored to veterinarians and physicians, who are key stakeholders for transmission prevention, in order to facilitate education of the public and raise awareness of echinococcosis. Ultimately, the project’s goal is to create a software supported framework for systematic surveillance of  Echinococcus to aid in the development of targeted transmission control actions to reduce echinococcosis case burden.

Тип пројекта:

National Projects

From Brain Waves to Memory Boost: Memory Enhancement by Personalized Frequency-Modulated Noninvasive Brain Stimulation

Project leader: Dr. Jovana Bjekić

Project collaborators: Dr. Katarina Vulić, Dr. Uroš Konstantinović, Dr. Saša Filipović, Dunja Paunović

Project number: 6058808

Duration of the project: 2020-2022.

Leading institution: Institute for Medical Research, University of Belgrade

Description of the project:

This project seeks to enhance the impact of transcranial direct current stimulation (tDCS) on memory by personalizing and optimizing the stimulation protocol for the parieto-hippocampal neural network. The project proposes an electrode positioning method that targets associative memory, a crucial function for remembering connected information. Unfortunately, associative memory deteriorates with age and is compromised in individuals with neurodegenerative conditions. The project also developed new frequency-modulated protocols and assessed their neurophysiological and behavioral effects. To further enhance the efficacy of the stimulation, the protocol was personalized to the individual theta rhythm. Theta rhythm is a neurophysiological marker of memory that appears in the electroencephalographic record when individuals attempt to remember or recall something. This project’s goal is to improve memory outcomes through customized and optimized tDCS protocols for the parieto-hippocampal neural network.

Тип пројекта:

National Projects

An Integrated Study of Regional Genetic and Environmental Risk Factors for Major Noncommunicable Diseases in the Serbian Population

Project leader: Dr. Dragan Alavantić

Project collaborators: Dr. Mirjana Gurinović, Dr. Marija Glibetić, Dr. Jasmina Martačić-Debeljak, Dr. Marija Takić

Project number: III 41028

Duration of the project: 2011-2020.

Leading institution: Vinča Institute of Nuclear Sciences

Description of the project:

Collaborators from IMR were engaged in the areas of research on nutragenomics and gene regulation of cardiovascular diseases.

Тип пројекта:

National Projects

Novel Encapsulation and Enzyme Technologies for Designing New Biocatalysts and Biologically Active Compounds Aimed at Enhancing Food Quality, Safety, and Competitiveness

Project leader: Dr. Branko Bugarski

Project collaborators: Dr. Vesna Ilić, Dr. Ivana Drvenica

Project number: III46010

Duration of the project: 2011-2019.

Leading institution: Faculty of Technology and Metallurgy, University of Belgrade

Description of the project:

Тип пројекта:

National Projects

Синтеза аминохинолина и њихових деривата као антималарика и инхибитора ботулинум неуротоксина

Води : академик др сци Богдан Шолаја

Сарадници на пројекту: Др сци Олгица Ђурковић Ђаковић

Број пројекта: OI 172008

Трајање пројекта: 2011.-2019.

Водећа институција:

Хемијски факултет Универзитета у Београду

Опис пројекта: 

Циљ :остварити замену антималарика хлорокина(ЦQ)због повећања броја МДР П.фалципарум сојева; развити нове тетраоксанске антималарике због појаве знатно смањене клиничке ефикасности лекова који садрже артемизиниске деривате (Нат.Рев.Мицробиол 2010,8,272); трансформисати водеће нетоксичне инхибиторе (СМНПИ) БоНТ/А ЛЦ у биорасположиве инхибиторе (ниска нМ област) који ће постати кандидати за лек. Развити једињења која би могла лечити ботулизам настао тровањем ботулинум неуротоксинима. Пројекат обухвата развој дуалних инхибитора,антималаријских једињења истовремено инхибитора ботулинум неуротоксина. Показано је да СМНПИ засновани на структури диазахризена, холне киселине и адамантана инхибирају ЦQР П.ф.сојеве са IC50 < 10nM, као и да лече мишеве при дозама од 50-80mg/кг/дан без токсичних ефеката после некропсије. Други антималарик, аминохинолински стероидни дериват показао се и као јак БоНТ инхибитор: БоНТ/А ЛЦ IC50 = 2.20µM;Ки = 3.20µM. Пројекат обухвата синтезу нових хемотипова и оптимизацију садашњих, код нас откривених водећих једињења (леадс); потенцијални ин виво кандидати биће тестирани на метаболичку стабилност у микрозомима јетре. Применићемо методе модерне рачунарске хемије,симулације понашања интерреагујућих молекула и израчунавања слике електростатичких потенцијала ових једињења у различитим пХ окружењима,примењујући више степене теорије. Научна сарадња са WРАИР (маларија), УСАМРИИД (инхибиција БоНТ) и САИЦ-НЦИ (компјутерско моделовање; ЦРАДА уговори и/или заједнички научни пројекти).
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